Intestinal superbacteria’C. Difficile’
U.S. Centers for Disease Control and Prevention regulates C. Difficile at the’Urgent’ level
Paper-based high-sensitivity multiple analysis enables diagnosis in less than 10 minutes
Researchers in Korea have developed a rapid kit that detects high-risk antibiotic-resistant super bacteria on-site.
On the 15th, the Korea Basic Science Institute (KBSI) announced that the research team of Dr. Choi Jong-soon of the Materials Analysis Research Department has developed a rapid detection kit that can detect the superbacteria Clostridioides difficile (hereinafter referred to as C. difficile).
C. difficile (Clostridioides Difficile) is an enteric bacteria that cannot be treated with antibiotics. It is a bacterium that causes severe diarrhea and colitis (inflammation of the colon). It is usually found in the environment, but most cases of C. difficile infection occur while taking antibiotics or shortly after taking antibiotics.
In severe cases, it can be accompanied by fulminant gastroenteritis, toxic giant colon, sepsis, etc., and can be life-threatening. The Centers for Disease Control and Prevention (CDC) stipulates C. Difficile as the minimum threat level’Urgent’ level. It is known that about 10% of elderly people 65 years of age or older who are infected with C. difficile die within a month.
In order to block the spread of C. difficile infection, which has no clear treatment so far, fast and accurate early diagnosis is required, but the currently used test method performs three steps, including the C. difficile antigen test, toxin test, and genetic test on the patient’s feces. It took a lot of time because it had to be done. In addition, the sensitivity of antigen and toxin tests is said to be very low.
KBSI’s Materials Analysis Research Department Dr. Choi Jong-soon, Han Do-kyung, and Biochemical Analysis Team, Dr. Gwen Joseph, conducted a joint study with the research team of Professor Dal-Sik Kim of the Department of Diagnostic Laboratory Medicine, Chonbuk National University Medical School. The researchers developed a high-sensitivity multiplex analysis technology that detects C. difficile and developed a paper-based multiple detection kit (mPAD) to which it was applied.
mPAD can be detected in 10 minutes with only one analysis, and a trace of low concentration C. Difficile sample can be detected in up to 1 hour through high-sensitivity signal amplification. The detection sensitivity of mPAD is 97%, the specificity is 88%, and the accuracy is 95%. In particular, the material is paper, and the manufacturing cost is low, and it is easy and friendly.
To operate mPAD, you can check whether C. difficile is detected by dropping fecal samples in hole S and water in hole B by heating the slide of the kit and closing the kit.
This research achievement is the result of KBSI’s biodisaster analysis technology development project, and the paper titled’Paper-based multiplex analytical device for simultaneous detection of Clostridioides difficile toxins and glutamate’ in Biosensors and Bioelectronics, the top academic journal in the field of analytical chemistry. It was published on the 15th as’dehydrogenase’.
Dr. Joseph Kwon said that this study is an example of securing the source technology for diagnosis of C. Difficile and suggests the possibility of localization. Through the results of this research, it will be possible to replace the existing high-cost test method by providing an accurate and inexpensive diagnosis method.
On the other hand, the treatment for C. difficile is still unknown, but according to the International Journal of Clinical Infectious Diseases, in 2019, a research team at Anne & Robert Troia Hospital in Chicago, USA found that exposure to C. difficile in infancy develops immunity. I have a bar.
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